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Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus Ciência Rural
Peixoto,Rodolfo de Moraes; Araújo,Renata de Moraes Peixoto; Peixoto,Luciana Jatobá e Silva; Reges,Ariel Marques; Alves,Ana Paula Pereira; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos; Costa,Mateus Matiuzzi da.
ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial culture; Intramammary infection; Small ruminant; Somatic cell count.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217
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Inhibition of the growth of major mastitis-causing pathogens by non-aureus Staphylococcus isolates using the cross-streaking method Arq. Bras. Med. Vet. Zootec.
Ferronatto,J.A.; Souza,F.N.; Libera,A.M.M.P. Della; Vliegher,S. De; Visscher,A. De; Piepers,S.; Blagitz,M.G.; Heinemann,M.B..
RESUMO O objetivo do presente estudo foi avaliar a capacidade de estafilococos não aureus (NAS) isolados de diferentes nichos ecológicos (leite, ambiente e ápice do teto), associados a vacas leiteiras, de inibir os principais agentes etiológicos da mastite bovina (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis e Escherichia coli). Neste estudo, 38 isolados NAS de diferentes nichos ecológicos foram avaliados quanto à capacidade de inibir o crescimento in vitro de importantes patógenos causadores de mastite pelo método cross-streaking. No total, 19 (50%) isolados de NAS (oito isolados de S. chromogenes, 10 de S. fleurettii e um de S. haemolyticus) apresentaram inibição contra os principais patógenos causadores de mastite. No entanto, a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dairy cows; Coagulase-negative staphylococci; Intramammary infection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501745
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Mastitis in the transition period: identification of potential blood markers Arq. Bras. Med. Vet. Zootec.
Santos,K.R.; Souza,F.N.; Blagitz,M.G.; Batista,C.F.; Bertagnon,H.G.; Gomes,R.C.; Molinari,P.C.C.; Diniz,S.A.; Silva,M.X.; Haddad,J.P.A.; Heinemann,M.B.; Libera,A.M.M.P.D..
ABSTRACT This study identified potential blood markers associated with mastitis in dairy cows under different dry therapies during the transition period, using a logistic regression model. Thirty-four Holstein dairy cows were divided into three groups: untreated controls (13 cows, 42 quarters); animals that received an antimicrobial at drying-off (10 cows, 40 quarters); and animals that were administered an internal teat sealant at drying-off (11 cow, 44 quarters). Blood and quarter milk samples were collected 60 days before the expected day of calving, on the day of calving, and three, seven, 15, 21, and 30 days after calving. Milk samples were submitted for bacteriological analysis and somatic cell count. Blood samples were collected for analyses of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intramammary infection; Risk factor; Drying-off; Periparturient period; Metabolism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401120
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